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. 2012 Apr;50(4):1178–1184. doi: 10.1128/JCM.06277-11

Fig 3.

Fig 3

Confirmation of the identity of O. viverrini genomic DNA (OvBD1, Vietnam; OvKK, Thailand) by loop-mediated isothermal amplification (LAMP) and PCR. A total of 100 ng of template was used in each case. (A) Products of mito-OvLAMP on 1.5% agarose stained with ethidium bromide. Lanes: M, 1-kb ladder marker (numbers on the left are sizes in base pairs); (-), negative control (no DNA); 1 and 2, LAMP products from templates of OvBD1 and OvKK, respectively. (B) Electrophoresis of PCR products of 216 bp (using primers F3 and B3) on 1% agarose. Lanes: M, 1-kb ladder marker; (-), negative control (no DNA) for templates, see Table 1): 1, OvBD1 (O. viverrini); 2, OvKK (O. viverrini); 3, OvDL3 (O. viverrini); 4, OvPY3 (O. viverrini); 5, OvQN (O. viverrini); 6, CsND (Clonorchis sinensis); 7, OvL (O. viverrini); 8, FhAU (Fasciola hepatica); 9, FgNB (F. gigantica); 10, HpuD2 (Haplorchis pumilio); 11, HtaTL (H. taichui).