Specificity assessment of the mito-OvLAMP assay by visualization on 1.5% agarose gel stained with ethidium bromide. Lanes: M, 1-kb ladder marker (numbers on the left are sizes in base pairs); (-), negative control (no DNA); (+), positive control (100 ng DNA template of O. viverrini); 1 through 9, LAMP reaction with DNA template (100 ng) from O. viverrini eggs, O. viverrini adult worm, O. viverrini single metacercaria, C. sinensis (CsND), Fasciolopsis buski (FbN), F. hepatica (FhAU), F. gigantica (FgNB), H. pumilio (HpuD2), and H. taichui (HtaTL).