Table 1.
Mean ratios for relative quantification of the B1 gene and AF146527 repeat element compared to the single-copy P30 gene in different strains of Toxoplasma gondiia
| Gene copy ratio | Type I |
Type II |
Type III |
P valueb | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Strain name | Mean (SD) ratio |
Strain name | Mean (SD) ratio |
Strain name | Mean (SD) ratio |
|||||
| Strain | Type | Strain | Type | Strain | Type | |||||
| B1/P30 | RH | 7.1 (0.7) | 7.3 (2.0) | B7 | 2.5 (0.0) | 2.9 (0.3) | STRL | 4.0 (0.2) | 4.9 (0.7) | 0.01 |
| VEL | 6.4 (0.4) | H44 | 2.7 (0.3) | VEG | 4.9 (0.3) | |||||
| ENT | 10.2 (0.8) | Me49 | 3.1 (0.2) | C5 | 5.8 (0.5) | |||||
| GT-1 | 5.7 (0.2) | PIH | 3.2 (0.1) | CTG | 4.9 (0.2) | |||||
| AF146527/P30 | RH | 30.4 (5.4) | 24.3 (4.2) | B7 | 52.3 (5.5) | 53.7 (10.2) | STRL | 49.1 (2.6) | 46.9 (6.2) | 0.02 |
| VEL | 23.8 (0.5) | H44 | 57.8 (3.2) | VEG | 53.7 (5.9) | |||||
| ENT | 21.8 (1.1) | Me49 | 40.3 (2.6) | C5 | 39.0 (2.4) | |||||
| GT-1 | 21.1 (0.6) | PIH | 64.3 (1.6) | CTG | 46.0 (4.8) | |||||
| AF146527/B1 | RH | 4.3 (0.7) | 3.5 (0.9) | B7 | 20.7 (1.9) | 18.8 (3.9) | STRL | 12.4 (1.0) | 9.9 (2.4) | 0.01 |
| VEL | 3.7 (0.3) | H44 | 21.3 (3.2) | VEG | 11.1 (1.9) | |||||
| ENT | 2.1 (0.2) | Me49 | 13.0 (0.3) | C5 | 6.8 (0.4) | |||||
| GT-1 | 3.7 (0.1) | PIH | 20.0 (0.6) | CTG | 9.4 (1.4) | |||||
a
T. gondii was harvested on human foreskin fibroblast culture, as described at http://sibleylab.wustl.edu/pdf/Toxo_Harvesting.pdf. Mean values are based on three independent experiments, with ratios calculated automatically using relative quantification software with qPCR efficiency specific for each T. gondii type.
b
Kruskal-Wallis rank sum test, with P < 0.05 considered significant.