Table 2.
Primers and probes used in the present study
| Primer or probe (GenBank accession no.) | Sequence | PCR product size (bp) | Modification |
|---|---|---|---|
| P30 gene (AY187278) | 126 | ||
| P30-1 | 5′-AGTTCCAATCGAGAAGTTCCC-3′ | None | |
| P30-2 | 5′-TTATTGACGACCGATGAGGC-3′ | None | |
| P30-3 | 5′-CAACCGACCACAAACGTCTGCG-3′ | 5′LCRed640 3′Ph | |
| P30-4 | 5′-CAACTCTGTGCGTCGTCTCCCTTGAT-3′ | 3′ FITC | |
| B1 gene (AF179871) | 132 | ||
| B1-1 | 5′-TCTCTCAAGGAGGACTGGCA-3′ | None | |
| B1-2 | 5′-GTTTCACCCGGACCGTTTAG-3′ | None | |
| B1-3 | 5′-ACGGGCGAGTAGCACCTGAGGAG-3′ | 5′LCRed640 3′Ph | |
| B1-4 | 5′-CGGAAATAGAAAGCCATGAGGCACTCC-3′ | 3′ FITCa | |
| AF146527 element (AF487550) | 133 | ||
| CG-1 | 5′-GTTGGGAAGCGACGAGAGTC-3′ | None | |
| CG-2 | 5′-ATTCTCTCCGCCATCACCAC-3′ | None | |
| CG-3 | 5′-TGCGATCTAGACGAGACGACGCTTTC-3′ | 5′LCRed640 3′Ph | |
| CG-4 | 5′-GGAGGGTGGAAAAGAGACACCGGA-3′ | 3′ FITC |
a
FITC, fluorescein isothiocyanate.