Fig 1.

PKAcatα and D-AKAP1 mRNA levels are decreased in P465L mutant carriers. (A) PKA subunit expression analysis of P465L mutant adipocytes from lean and obese mice versus mature adipocytes isolated from 12- to 16-week-old wt counterparts. (B) Protein levels of PKA subunits of P465L mutant adipocytes from lean and obese mice versus mature adipocytes isolated from wt counterparts. (C) akap-1 expression analysis of P465L mutant adipocytes from lean and obese mice versus mature adipocytes isolated from 12- to 16-week-old wt counterparts. The data represent means ± standard errors (SE) for 6 to 8 animals per group. Fold changes were established using the wt as 100. Differences in expression between groups were assessed by analysis of variance (ANOVA) (G, effect of P465L mutation; OB, effect of obesity; G × OB, interactive effect) and the Student t test. *, P465L versus wt or P465L/ob/ob versus ob/ob; °, wt versus ob/ob or P465L versus P465L/ob/ob. The level of probability was set at a P value of <0.05.