Fig 3.

(A) atgl mRNA levels are decreased in P465L mutant carriers. Shown is lipase expression analysis of P465L mutant adipocytes from lean and obese mice versus mature adipocytes isolated from 12- to 16-week-old wt counterparts. The data represent means ± SE for 8 to 12 animals per group. Fold changes were established using the wt as 100. Differences in expression between groups were assessed by ANOVA and the Student t test. *, P465L versus wt or P465L/ob/ob versus ob/ob; °, wt versus ob/ob or P465L versus P465L/ob/ob. The level of probability was set at a P value of <0.05. (B) HSL and ATGL protein levels are decreased in P465L carriers. Shown are the results of Western blotting of ser660-HSL (Tot HSL), ser565-HSL HSL, and ATGL. Differences in expression between groups were assessed by ANOVA (G, OB, and G × OB) and the Student t test. *, P465L versus wt or P465L/ob/ob versus ob/ob. The level of probability was set at a P value of <0.05.