Fig 5.

(A) P465L carriers are more sensitive to the antilipolytic action of insulin. Dose-response curves show the inhibitory effect of insulin on the induced glycerol release in isolated mature white adipocytes from wt and P465L adipocytes in lean and obese mice treated with NA. (B) Increased levels of AKT in P465L carriers. Shown are the effects of the selective phosphodiesterase inhibitors cilostamide (PDE3) and rolipram (PDE4) on the lipolytic inhibition elicited by insulin in the presence of NA in lean mice. (C) PDE proteins are not responsible for the increased sensitivity to the antilipolytic action of insulin in P465L carriers. Shown are the results of Western blotting of Ser473-AKT and total AKT. Differences in expression between groups were assessed by the Student t test. The level of probability was set at a P value of <0.05.