Table 1.
Effect of P465L mutation on catecholamine-induced lipolysis in isolated white mature adipocytes from lean and obese mice (females 12 to 16 weeks old)a
| Treatment | Maximal response (Emax) |
EC50 (nM) |
||||||
|---|---|---|---|---|---|---|---|---|
| Wt | P465L | ob/ob | P465L × ob/ob | Wt | P465L | Wt | P465L × ob/ob | |
| Noradrenaline | 302.5 ± 22.6 | 222.7 ± 25.8b | 103.5 ± 13.5 | 68.5 ± 12.2c | 9.8 ± 3.4 | 53.0 ± 29.8 | 10.8 ± 6.4 | 91.4 ± 88.5 |
| CGP 12177A | 113.6 ± 9.7 | 79.3 ± 8.0b | 93.4 ± 12.3 | 54.1 ± 9.9c | 509 ± 154 | 509 ± 154 | 122.3 ± 69.2 | 126.0 ± 107.9 |
| Forskolin | 123.5 ± 19.9 | 124.4 ± 16.5 | 242.2 ± 27.9 | 138.1 ± 14.5c | 8.1 ± 5.9 | 48.8 ± 33.1 | 1,330.0 ± 399.8 | 1,330.0 ± 399.8 |
| DBcAMP | 197.0 ± 19.3 | 197.0 ± 19.3 | 86.78 ± 11.3 | 86.78 ± 11.3 | 226.6 ± 118 | 53,078 ± 30,540b | 272.2 ± 212 | 3,801.2 ± 3,045.0c |
| Basal | 100 ± 8.5 | 85 ± 9.4 | 100 ± 10 | 83.8 ± 8.7 | ||||
a
The data are means ± standard errors. Lipolytic activity is expressed as a percentage of stimulation over basal lipolysis; a value of 100 was set up as a control for wt and ob/ob mice. Maximal action over basal lipolysis and significant differences were obtained using the GraFit computer program.
b
Versus the wt.
c
Versus ob/ob.