Fig 3.
RCF1 and RCF2 genetically interact and together are required to support the COX enzyme activity. (A) Serial 10-fold dilutions of wild-type (WT), Δrcf1, Δrcf2, or Δrcf1 Δrcf2 cells were spotted on YP plates containing glucose (YPD) or glycerol (YPG) and grown at 30°C. (B) The COX and cytochrome bc1 relative specific enzyme activities were determined and expressed as a percentage of the value for the wild-type control. The averages of three replicate measurements are given. (C) Serial 10-fold dilutions of wild-type (WT) cells and Δrcf1 Δrcf2 cells expressing Rcf1His or Rcf2His or not (−) were spotted on YP plates containing glucose (YPD) or glycerol supplemented with 0.1% galactose (YPG + 0.1% Gal) and grown at 30°C.