Fig 7.

Impact of PN-1 deficiency on ex vivo angiogenesis. Thoracic aortic rings from PN-1-deficient (-/-) and WT (+/+) mice were cultured in a collagen gel in the presence or absence of 50 ng/ml VEGF and 20 μg/ml recombinant WT PN-1. The microvascular networks sprouting from the rings were observed by phase-contrast microscopy and after isolectin B4 labeling (in red), and results were quantified by densitometric analysis. Representative micrographs and results are shown for 6 to 20 rings from 3 to 7 mice. ***, P < 0.001 WT versus PN-1-deficient mice in the presence or absence of recombinant PN-1 (rPN-1).