Fig 1.

DENSPM-induced translation of recombinant His-tagged SSAT. The top panels show semiquantitative RT-PCR comparing SSAT RNA of control HEK293T cells and that of cells stably transfected with a plasmid containing the SSAT ORF with a His tag (HEK 293T-SSAT). The bottom panels show anti-His tag Western blots of the same cells. Actin served as the target and loading controls. Transfection alone markedly increased SSAT RNA, but the SSAT protein remained undetected. A 12-h exposure to 10 μM DENSPM, a polyamine analogue and potent inductor of SSAT translation, strongly induced the His-tagged SSAT protein with little or no further increase in SSAT RNA.