Fig 3.

Nucleolin-SSAT RNA interaction in situ. Protein-RNA coprecipitation was detected using RT-PCR. Lanes 1 to 4 are products of RT-PCRs. Lanes 5 to 8 are negative controls omitting reverse transcriptase. Lanes 1 and 5 are positive controls with the PCR target being whole-cell lysate. Lanes 2 and 6 are negative controls with the PCR target being sham immunoprecipitate from protein G-coated beads without bound antibody (No Ab). Lanes 3 and 7 are negative controls with the PCR target being immunoprecipitate using antiactin monoclonal antibody. Lanes 4 and 8 used immunoprecipitate from beads with antinucleolin monoclonal antibody as the PCR target. The data show SSAT RNA coprecipitates with nucleolin.