Fig 4.

Glycine/arginine-rich nucleolin domain is required for SSAT mRNA binding. (A) Nucleolin schematic with amino acid numbers: N-term, N-terminal region; RRM1 to RRM4, “RNA recognition motif” domains 1 to 4; GAR, glycine/arginine-rich domain. (B) Western blots using an anti-His tag monoclonal antibody confirmed expression of nucleolin fragments by cells transiently transfected with plasmids containing the indicated constructs (native nucleolin or fragments not detected). (C) Anti-His tag Western blots of SSAT-RNA column eluates from transiently transfected cell lysates show that the binding does not occur without the GAR domain.