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Characterization of 55-kDa nucleolin isoform by mass spectroscopy. The nucleolin isoform interacting with SSAT RNA was separated by 2DE, trypsin digested, and analyzed by mass spectroscopy. The tandem mass spectrometry (MS/MS) profile of each the four identified peptides (panels A to D) and their representative sequence is shown on the right of each panel. The location of each peptide is shown in panel E.
