Med1+/−
Med24+/− mammary gland development is retarded during puberty. (A, B, and C) Whole-mount staining of virgin female mammary inguinal glands at the ages of 6 and 8 weeks is shown. (A) Med1+/−
Med24+/+; (B) Med1+/+
Med24+/−; (C) Med1+/−
Med24+/−. Scale bar, 1 mm. (D) The number of duct branches per visual field is shown. The Med1+/−
Med24+/− glands show an attenuated number of duct branches. Values are means ± standard deviations (SD). *, P < 0.05; **, P < 0.01. n = 2, 4 weeks old; n = 6, 6 weeks old; n = 10, 8 weeks old.