Fig 11.

Transcriptional functions of MED1 and MED24 for effects on proliferation of ER-negative BT-549 cells. αMED1, anti-MED1; αTBP, anti-TBP. The full-length proteins or fragments of MED1 (A to C) or MED24 (D to F) were tested to rescue the effects of knockdown of MED1 or MED24. (A and D) Western blot analyses 2 days after transfection. (B and E) BrdU incorporation 2 days after transfection. (C and F) Numbers of cells after transfection. Values are means ± SD from a representative experiment performed in triplicate (*, P < 0.05; **, P < 0.01). Values are plotted as fold increase against the value from cells transfected with control vector and siRNA (B and E).