Fig 5.

RalB suppression and impaired invadopodium formation are not associated with reduced MMP-2/MMP-9 gelatinase activity or MT1-MMP plasma membrane localization. (A) Representative zymograms from 24-h-conditioned medium showing secreted MMP-2 and MMP-9. (B) Representative Western blot analysis of MT1-MMP in input lysates and avidin pulldown following surface biotinylation in CFPAC-1 cells stably expressing control GFP (GFPi) or RalB-specific shRNA (RalBi). Data shown are representative of two independent experiments.