Fig 3.

PALB2 binds KEAP1 through a highly conserved ETGE motif. (A) Schematic of PALB2 constructs used in the domain mapping study. (B and C) PALB2 constructs were transiently expressed in 293T cells, proteins were IPed with anti-FLAG M2 agarose, and the precipitates were analyzed by Western blotting. (D) Amino acid sequence alignment of the N-terminal sequences of PALB2 and NRF2 showing the shared LDEETGE KEAP1 binding motif. (E) Deletion or mutations of the ETGE motif in PALB2 all abolish KEAP1 binding. (F) E91R and T92E mutations do not affect the binding of PALB2 to BRCA2, RAD51, and BRCA1.