Fig 6.

siRNA-mediated depletion of PALB2 reduces NRF2 abundance and activity in the nucleus and increases cellular ROS level. (A) Western blotting showing the levels of proteins of interest after siRNA treatments. (B) ROS levels in cells depleted of each of the indicated proteins. (C) Endogenous NRF2 activity on an ARE-luciferase reporter following depletion of PALB2 and other proteins. (D and E) Nuclear NRF2 level after depletion of PALB2 in U2OS cells analyzed by Western blotting (D) and quantified by densitometry (E). (F and G) ChIP analysis of endogenous NRF2 binding to NQO1 ARE in control and PALB2-depleted cells. (F) An agarose gel image of the PCR products in a representative experiment. (G) Results quantified by real-time PCR.