Fig 8.
Dual-modality immunization with gE2-del virus and gC2 and gD2 subunit antigens. Guinea pigs were either mock immunized or immunized i.m. twice with 5 × 105 PFU gE2-del virus plus gC2 and gD2 subunit antigens and were challenged IVAG with 5 × 105 PFU HSV-2 MS. (A) Survival kinetics. (B) Vaginal disease scores for surviving animals only. (C) Vaginal viral titers. Results in panel B represent means ± SEM, while those in panel C represent geometric means ± SEM for 5 animals in the mock-immunized group and 10 animals in the gE2-del–gC2–gD2-immunized group. (D) Recurrent vaginal shedding of HSV-2 DNA at 28 to 48 days postinfection. The table summarizes the number of episodes of shedding of 151 to 1,000 copies, 1,001 to 100,000 copies, or >100,000 copies of HSV-2 Us9 DNA.