Fig 3.

Reactivity of vtHBsAg in immunoassays based on three monoclonal anti-HBs antibodies. The culture supernatants (A and C) and cell lysates (B and D) of transiently transfected cells expressing wt- and vtHBsAgs were collected at 48 h. wt- and vtHBsAgs were detected by ELISAs based on three monoclonal anti-HBs antibodies, A11, S11, and S1. The reactivity of vtHBsAgs with the amino acid substitutions at position 145 (A and B) or 122 (C and D) was expressed as the percentage of the OD₄₅₀ of samples compared to that of the wtHBsAg. The average values from 4 replicates were calculated and given as the final reactivity of wt or vtHBsAgs. CK, cells transfected with empty plasmid as a negative control.