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IF staining of wt- and vtHBsAgs with amino acid substitutions with anti-HA antibody. HeLa cells were transfected with the expression plasmid pHBsAgWT, pHBsAgG145X, or pHBsAgK122X, respectively. Cells were fixed 48 h after transfection and stained with a monoclonal mouse antibody to the HA tag. The nuclei were stained with Hoechst 33258. Magnification, ×400. CK, cells transfected with empty plasmid as a negative control.
