Table 1.

Summary of characteristics of vtHBsAgs^a

Group and amino acid change	NTb	SS	Antigenicity	Anti-HBsc	CHd	Molecular size (kDa)	PI	SC
Glycine
K122G	GGA	++++	++++	ND	ND	75.07	5.97	4
WT (G145)	GGA	++++	++++	++++	++++	75.07	5.97	4
HpB
G145 M	ATG	++++	+++	ND	ND	149.21	5.75	1
G145L	TTA	+++	++	ND	ND	131.17	5.98	6
G145I	ATA	+++	+	+	+++	131.17	6.02	3
G145P	CCA	+++	+	ND	ND	115.13	6.3	4
WT (K122)	AAA	++++	++++	++++	++++	146.19	9.74	2
K122 M	ATG	++++	++++	ND	ND	149.21	5.75	1
K122L	TTA	++++	++++	+++	++++	131.17	5.98	6
K122I	ATA	+	+	+	+	131.17	6.02	3
HpL
G145N	AAC	+++	+++	ND	ND	132.1	5.41	2
G145Q	CAA	++++	+++	ND	ND	146.15	5.65	2
G145T	ACA	++++	+++	ND	ND	119.12	6.53	4
K122P	CCA	++++	++++	ND	ND	115.13	6.3	4
K122N	AAT	++++	++++	ND	ND	132.1	5.41	2
K122Q	CAA	++++	++++	ND	ND	146.15	5.65	2
Negative charge
G145D	GAC	++++	+++	ND	ND	133.1	2.97	2
G145E	GAA	++++	+++	++	+	147.13	3.22	2
K122T	ACA	++++	++++	ND	ND	119.12	6.53	4
K122D	GAT	++++	++++	ND	ND	133.1	2.97	2
K122E	GAA	++++	++++	ND	ND	147.13	3.22	2
Indolyl
G145W	TGG	+	++	+	+	204.22	5.89	1
K122W	TGG	+++	++++	ND	ND	204.22	5.89	1
Positive charge
G145H	CAC	++++	+++	ND	ND	155.16	7.59	2
G145R	CGA	+++	+++	+	++	174.2	10.76	6
K122H	CAT	++++	++++	ND	ND	155.16	7.59	2

^aHpB, hydrophobicity; HpL, hydrophilicity; NT, nucleotide; SS, secretion of HBsAg; CH, HBsAg clearance; PI, isoelectric point; SC, synonymous codons. ++++, Normal; +++, slightly impaired; +, impaired; ND, not detected.

^bThe nucleotide substitution.

^cThe ability to induce anti-HBs.

^dThe ability to clear wtHBsAg.