Fig 1.

Intracellular cytokine staining of PBMC for SIV-specific CD4⁺ and CD8⁺ memory T cells secreting IFN-γ, IL-2, and TNF-α. (A to D) Stacked responses to SIV Env and Gag peptide pools by CD4⁺ and CD8⁺ CM and EM cells over the course of immunization. Dashed arrows mark the Ad recombinant administrations at weeks 0 and 12. Solid arrows mark the gp120 protein boosts at weeks 24 and 36. In panel A, *** signifies P values across the four immunization groups of 0.0006 for Env, 0.0023 for Gag, and 0.0005 for the sum of both. In panel B, *** signifies P values across the four immunization groups of 0.0092 for Env, 0.0006 for Gag, and 0.0006 for the sum of both.