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Mutational effects on viral entry as determined by measuring luciferase activity in two different cell lines. (A) Relative luciferase units (RLU) for 293T target cells. (B) RLU for A549 target cells. HIV NA pseudotyped with only the NA glycoprotein was used as a negative control. VSV-G pseudotyped viruses served as a positive control. The error bars are the standard deviations (SD) of three independent experiments from the transfection step.
