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Growth proprieties of recombinant influenza HAlo viruses expressing parental HA and mutant Y161A or K307A/K310A proteins. MDCK cells were infected at an MOI of 0.01 with the different recombinant viruses. Viruses released to the supernatant were titrated at 0, 12, 24, 48, and 60 h postinfection by plaque assay on fresh MDCK cells.
