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Generation and replication of VSV-MSC69A. (A) The gene encoding MSC69A inserted in the fifth position of the VSV genome, diagrammed from the 3′-to-5′ orientation of the negative-stranded viral RNA genome. (B) BHK cells were infected with VSV-MS or VSV-MSC69A, and cell culture medium was collected 2, 4, 6, and 8 h postinfection. Standard plaque assays were performed to measure virus replication.
