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. 2012 Apr;86(8):4253–4261. doi: 10.1128/JVI.05991-11

Fig 3.

Fig 3

Specific T cell responses are elicited following a single immunization with VSV-MS. (A) CB6F1 mice were immunized with VSV-MS (n = 9) or VSV-MSC69A (n = 10). Seven days postimmunization, splenocytes were harvested and analyzed using an IFN-γ ELISPOT assay. (B) A second set of CB6F1 mice was immunized with VSV-MS (n = 8) or VSV-MSC69A (n = 7). Thirty days postimmunization, splenocytes were harvested and memory responses were analyzed using an IFN-γ ELISPOT assay. Two peptides corresponding to known HBV-specific, H-2d-restricted epitopes (designated 191 and 364); two peptides corresponding to known HBV-specific, H-2b-restricted epitopes (designated 353 and 371); and a VSV N peptide were used to stimulate splenocytes in the ELISPOT assays. (C) CB6F1 mice were boosted 3 weeks after pCMV-MS (n = 3) or pCMV-MSC69A (n = 4) prime with VSV-MS or VSV-MSC69A, respectively. Fourteen days postboost, CD4 T cells were purified and stimulated in an IFN-γ ELISPOT assay to measure the response. The number of cells responding to stimulation is represented as a quantification of the number of SFC/106 cells. All values are presented as the average of each group; error bars represent standard errors.