Fig 4.
HBV-specific antibody responses following VSV-MS immunization are independent of MS secretion. (A) CB6F1 mice were immunized with various inocula (106, 104, or 102 PFU) of VSV-MS (n = 15) or VSV-MSC69A (n = 15), and unimmunized mice were used as controls (0.8 U/liter; data not shown). A quantitative HBsAb ELISA was conducted on serum collected 30 and 60 days postimmunization to measure specific antibody titers. (B) CB6F1 mice were immunized with 106 PFU of VSV-MS (n = 7) or VSV-MSC69A (n = 8); unimmunized mice were used as controls (n = 7). A qualitative HBsAb isotype-specific ELISA was conducted on serum collected 30 days postimmunization to determine isotype-specific antibody titers. Background (fetal bovine serum plus antibody) has been subtracted from represented values.