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. 2012 Apr;86(8):4527–4537. doi: 10.1128/JVI.07071-11

Fig 7.

Fig 7

DDX41 but not p204 contributes to the rAdV recognition response. (A) wt RAW 264.7 cells infected with sh-SC (scrambled nontargeting) or shP204 (hairpin targeting p204) were mock or Ad5CiG infected (20,000 p/cell). Cell lysates were harvested at the indicated time points and analyzed by Western blotting. (B) wt RAW 264.7 cells infected with sh-SC (scrambled nontargeting) or shDDX41 (hairpin targeting DDX41) were mock or Ad5CiG infected (20,000 p/cell). Cell lysates were harvested at the indicated time points and analyzed by Western blotting. (C) RNA isolated from scrambled- or shP204-uninfected cells was characterized for knockdown of P204 mRNA by a two-step qRT-PCR assay. RNAs were normalized to total HPRT mRNA by the ΔΔCT method as described in Materials and Methods. (D) Two-step qRT-PCR assay of mRNA harvested from scrambled- or sh-DDX41-infected cells, measuring relative levels of DDX41 mRNA normalized to HPRT mRNA.