Fig 6.

Effects of nonsense mutations on frameshifting. (A) Table summarizing the names of viruses, sequences surrounding the G string in the N-terminal reading frame (underlined codons are introduced stop codons, and the G strings are in bold), expected sizes of N-terminal products, and TK activity (+, detectable; −, not detectable), as shown by plaque autoradiography (data not shown). (B) Western blot analysis of the expression of the N-terminal products using anti-TK antibody (top) and ICP8 using anti-ICP8 antibody (bottom). The viruses used (12 hpi, MOI = 10) are indicated at the top. The molecular mass numbers to the left are derived from molecular mass markers run alongside the samples. (C) IP-Western (FLAG) analysis of the expression of full-length TK in G8-FLAG- and nonsense mutant-infected cells (12 hpi, MOI = 10). The names of the viruses are indicated at the top.