Fig 1.

CMV-tax/rex-LTR and pc-Tax express both Tax and Rex. (A) Schematic representation of tax/rex RNA, pc-Tax, and the CMV-tax/rex-LTR construct and detection of Tax expression from CMV-tax/rex-LTR and pc-Tax. 293T cells were transfected with 4 μg of pc-Tax or CMV-tax/rex-LTR plasmid. Forty-eight hours after transfection, cell lysates were prepared and subjected to Western blotting. (B) Sequence comparison between wild-type tax/rex, pc-Tax, and pc-Tax (Rex−). (C) 293T cells were transfected with 4 μg of pc-Tax, pc-Tax (M22), pc-Tax (M47), or pc-Tax (K88). Tax and Rex were detected by Western blotting.