Skip to main content
. 2012 Apr;86(8):4222–4233. doi: 10.1128/JVI.06399-11

Fig 2.

Fig 2

Characterization of 55R E1A antiserum. (A) Western blot and Ponceau staining of GST-purified 55R E1A (or 53R E1A, in the case of HAdV-12), obtained using rabbit polyclonal anti-55R E1A Abs. Upper bands of Ponceau staining correspond to E1A proteins. (B) EGFP-55R E1A was immunoprecipitated from HT1080 cells by use of rabbit polyclonal anti-55R E1A Abs. (C) Indirect immunofluorescence images of HT1080 cells expressing myc-55R E1A or left untransfected and stained with an anti-myc Ab and anti-55R E1A polyclonal Abs. Nuclei were stained with Hoechst 33342.