Table 1.
Primers used in real-time RT-PCR
| Primer | Sequencea |
|---|---|
| Total LMP-1 | 5′-GCAGGAGGGTGATCATCAGT-3′ |
| 5′-GTCCTCTATTCCTTTGCTCTCATG-3′ | |
| 3.5-kb LMP-1 | 5′-TACGTAGCCGCCCTACATAAG-3′ |
| 5′-CCTCTCAAGGTCCAGGTCCAT-3′ | |
| LMP-2A | 5′-CAGGCAGGCATACTGGATTC-3′ |
| 5′-CGATGAGGAACGTGAATCTAATG-3′ | |
| EBNA-2 | 5′-GGACACAAGAGCCATCACCT-3′ |
| 5′-CAAAGCATTCGCATAGCAGA-3′ | |
| ISG56 | 5′-GTGACATCTCAATTGCTCCAGAC-3′ |
| 5′-GGAGCCTGGCTAAGCAAAAC-3′ | |
| GAPDH | 5′-GGAAGGTGAAGGTCGGAGTCA-3′ |
| 5′-ATGGGTGGAATCATATTGGAACA-3′ |
a
Sequences of forward and reverse primers are provided. The LMP-1 coding and regulatory regions containing the primer binding sites were sequenced in Daudi cells. Primers (Sigma-Aldrich) for LMP-1 were designed on the basis of these sequences.