Fig 5.

Cell fusion activity when BV glycoproteins are replaced by homologous glycoproteins from HSV-1 and CeHV-2. Target CHO cells were transfected with pCDNA3 (empty vector), PILRα, or nectin-1, along with a reporter plasmid expressing luciferase under the control of the T7 promoter. The transfected cells were replated with effector CHO cells transfected with a complete set of BV glycoproteins, or glycoproteins were replaced by gB, gD, gH, gL, or gH/gL from CeHV-2 and HSV-1, along with plasmids expressing T7 polymerase. Relative cell fusion activity of each replacement is presented as a percentage of that of the complete BV glycoprotein set required for fusion of nectin-1. Each bar shows the mean and standard deviation of three independent determinations. The data shown are representative of the complete data set obtained by the glycoprotein substitutions as shown in Table 2.