Fig 3.
Integration of rAAVs into genomic DNA. (a) PCR amplification of different mouse iPSC clones to detect genomically integrated rAAV vectors. (b) Genomic integrations further confirmed by Southern blotting. Genomic DNA was digested with an enzyme cutting approximately 500 bp downstream from the 3′ end of AAV and detected using a probe recognizing the left ITR and part of the CAG promoter. The first 12 clones, from clone 5 to clone 2-2-20k, were induced at an MOI of 2 × 104, and the rest of the clones were induced at an MOI of 2 × 105.