Fig 7.
The HCoV-229E Nsp7-10 polyprotein is a de novo RdRp in vitro. (a) Purified HCoV-229E Nsp7-10 polyprotein (52.5 kDa) analyzed by SDS–15% PAGE. (b) RdRp activity of HCoV-229E Nsp7-10 polyprotein in the presence or absence of Mn2+.
The HCoV-229E Nsp7-10 polyprotein is a de novo RdRp in vitro. (a) Purified HCoV-229E Nsp7-10 polyprotein (52.5 kDa) analyzed by SDS–15% PAGE. (b) RdRp activity of HCoV-229E Nsp7-10 polyprotein in the presence or absence of Mn2+.