Table 4.
Average cell numbers and relative frequency of leukocyte populations in the lung in SARS-CoV infection
| Cell type | No. of cellsa |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Mock infection | Primary infection |
Rechallenge |
|||||||
| Day 1 | Day 3 | Day 5 | Day 10 | Day 14 | Day 28 | Day 5 | Day 28 | ||
| Leukocytes | 4.4E6 ± 1E6 | 7.9E6 ± 1E6 | 1.2E7 ± 4E6 | 6.7E6 ± 1E6 | 1.1E7 ± 4E6 | 2.8E7 ± 8E6* | 6.0E6 ± 9E5 | 6.64E6 ± 7E5 | 5.88E6 ± 1E6 |
| T cells | 69.2 ± 4.4 | 64.1 ± 5.2 | 64.7 ± 3.6 | 76.5 ± 1.2 | 65.4 ± 4.6 | 35.7 ± 5.3* | 52.4 ± 4.2 | 58.9 ± 2.6 | 45.6 ± 4.2 |
| B cells | 1.5 ± 0.2 | 4.1 ± 0.3 | 2.4 ± 0.5 | 4.1 ± 0.6 | 3.4 ± 0.5 | 3.7 ± 0.9 | 2.2 ± 0.6 | 3.2 ± 1.2 | 4.2 ± 0.5* |
| aMϕ | 6.6 ± 3.1 | 14.4 ± 2.4 | 16.4 ± 4.1 | 17.2 ± 2.9 | 12.1 ± 2.2 | 25.1 ± 5.7* | 18.2 ± 3.3 | 5.3 ± 1.1 | 5.0 ± 1.3 |
| mDCs | 8.2 ± 1.4 | 7.1 ± 2.1 | 11.3 ± 1.9 | 14.0 ± 3.4 | 8.1 ± 1.1 | 16.1 ± 2.1 | 8.3 ± 2.9 | 8.6 ± 0.8 | 11.3 ± 1.7 |
| Eosinophils | 1.9 ± 1.0 | 3.7 ± 0.4 | 8.7 ± 3.4 | 2.1 ± 1.0 | 2.8 ± 0.1 | 4.0 ± 0.8 | 5.1 ± 1.0 | 5.8 ± 1.0 | 9.3 ± 0.9* |
a
Average absolute numbers of leukocytes per gram tissue ± SEM and the average frequency of T cells (CD3+), B cells (CD20+), aMϕ (CD14+ CD11c+), mDCs (CD14−CD11c+), and eosinophils (CD14−FcεR1+CD23+) of total lung leukocytes as determined by flow cytometry. Asterisks indicate values that are significantly different from those of mock-infected controls (P < 0.05).