Skip to main content
. 2012 Apr;86(8):4182–4193. doi: 10.1128/JVI.07105-11

Fig 6.

Fig 6

EIAV NC mutants are budding defective. Coexpression of EIAV L domain mutant eYP−, which harbors a functional NC, rescues the release of the eRKI and eRKII NC mutants. 293T cells were transfected with eWT (lane 1), eYP− (lane 2), eRKI (lane 3), or eRKII (lane 7) alone or cotransfected with the indicated NC mutant and eYP− plasmids (lanes 4, 5, 6, 8, 9, and 10). The transfected DNA ratios of the indicated NC mutant and the YP mutant were 4:1, 1.5:1, and 1:4 while the total amount of DNA remained constant. Viral fractions and cell lysates were analyzed by Western blotting using the indicated antibodies. Relative virus release efficiencies are shown (± the standard deviations; n = 3). (B) EIAV NC mutants displayed typical budding defects in 293T cells. Shown are transmission EM images of thin-sectioned 293T cells transfected with WT EIAV (a and b) or the eRKI (c and d) or eRKII (e and f) mutant. Arrows indicate either WT mature virions (a and b) or tethered arrested particles (c to f). Scale bars, 200 nm.