Fig 6.

Dimerization of N proteins in extracellular virions. Virions from culture supernatants were purified in the absence or presence of NEM as described in Materials and Methods. Purified particles were resolved by SDS-PAGE under reducing or nonreducing conditions and subjected to Western blot analysis. (A) Purified wild-type and mutant APRRS particles without NEM treatment. (B) Purified wild-type and mutant SHE particles without NEM treatment. (C) Purified APRRS and SHE particles with NEM treatment. Monomers (N) or dimers (2N) of the N protein are indicated by arrows.