Fig 2.

Viral replication in flu-infected WT and DKO mouse lungs and TEC cultures. (A) Mice were infected intranasally with 2,000 EID₅₀ of PR8. Lungs were isolated at d3 (WT mice, n = 15; DKO mice, n = 16) and at d7 (WT mice, n = 18; DKO mice, n = 15). Viral titers in the lungs were determined by plaque-forming assay with MDCK cells. Data are from three independent experiments (mean and SEM). (B) WT and DKO mouse TECs were infected with PR8 at a multiplicity of infection of 0.01 in triplicate. Apical supernatants were collected at 16, 48, 72, and 96 h postinfection and assessed for viral titer by plaque-forming assay with MDCK cells. Data are from three independent experiments (mean and SEM).