Skip to main content
. 2012 Apr;86(8):4631–4643. doi: 10.1128/JVI.06265-11

Fig 6.

Fig 6

Requirement for compatibility between the 5′-UTR-located inter-stem-loop and downstream partnering domains for MHV replication. (A) For viability, the chimeric virus must contain the MHV inter-stem-loop domain within the BCoV 5′ UTR when in the MHV background (as in B1–141,174-226/M), the BCoV inter-stem-loop domain and BCoV Nsp1 coding region when in the MHV background (as in B142–173,227-906/M), or the BCoV 5′ UTR and BCoV Nsp1 coding region when in the MHV background (as in B1–906/M). Note that the in-frame C-terminal fusion site for the Nsp1 coding region occurs between BCoV nt 906 and MHV nt 909. (B) Plaque phenotypes for the progeny from the variant constructs described for panel A. (C) Growth kinetics of the progeny depicted in B. (D) Northern analyses of the progeny depicted in for panel B.