Fig 1.

AS601245 inhibits reactivation of latent HIV-1 infection. (A) In a dose matrix experiment, TNF-α as the HIV-1-reactivating agent and AS601245 were titrated on the latently HIV-1-infected CA5 T cells. Levels of HIV-1 reactivation were determined 24 h poststimulation using flow cytometric analysis (% GFP-positive cells). TNF-α concentrations (ng/ml) are indicated. (B) To determine whether the inhibitory effect of AS601245 on HIV-1 reactivation was dependent on the type of viral integration, we determined the influence of increasing concentrations of AS601245 on PMA-mediated HIV-1 reactivation (10 ng/ml) in latently HIV-1-infected T cells in which HIV-1 was integrated in the same sense orientation relative to the transcriptional orientation of the host gene (CA5 cells), T cells in which HIV-1 was integrated in the converse sense orientation relative to the host gene (EF7 cells), and T cells in which HIV-1 was integrated into an intergenic region (CG3 cells).