Fig 2.

AS601245 inhibits HIV-1 reactivation in primary T cells. Latently HIV-1-infected primary T cells were generated as previously described (5). (A) PBMCs from donor 1 were infected with a GFP reporter virus. Active background infection (Control) and the level of reactivated HIV-1 were determined by flow cytometric analysis for GFP expression. HIV-1 reactivation was achieved by antibody-mediated CD3/CD28 costimulation. Cyclosporine (CsA), a reported inhibitor of CD3/CD28-mediated HIV-1 reactivation, was used as a specificity control. The inhibitory effect of AS601245 (AS) at 10 μM on HIV-1 reactivation is shown in the last dot plot. (B) The same experiments were performed using HIV-1 HXB2, and infection levels were detected by intracellular staining for Gag p24 protein. (C) FSC/SSC (cell size/granularity) plots reveal the resting phenotype prior to stimulation and the transition to an activated cell phenotype following CD3/CD28 stimulation in the presence or absence of cyclosporine or AS601245.