Fig 4.

AS601245 forces HIV-1 into latent infection. To determine whether AS601245 would promote the establishment of latent HIV-1 infection, Jurkat T cells were infected with a GFP reporter virus (NLENG1) at different MOIs (6 to 47% active infection on day 3) either in the absence (Control) or presence of AS601245. (A) On day 17 postinfection, each culture was split, and the subcultures were either left untreated (C) or were stimulated with PMA to trigger (re)activation of silent HIV-1 infection events. The level of active HIV-1-expressing cells in either culture was then determined after 24 h as the percentage of GFP-expressing cells. For each culture condition, the difference in the percentage of GFP-positive cells in the control culture and the corresponding PMA-induced cultures is equal to the size of the formed reservoir of latently HIV-1-infected T cells. To account for the differences in initial infection, results are represented as relative infection levels. Results represent the means ± standard deviations from four independent experiments. To determine the significance of the observed differences, we used Student's t test. (B) Cell viability for the respective experimental conditions.