Fig 5.

Effect of AS601245 on cellular gene expression. (A) PBMCs were left untreated (Control), treated with 10 μM AS601245, or activated with an anti-CD3/CD28 MAb combination in the absence or presence of 10 μM AS601245. After 24 h, the influence of AS601245 on cell activation as defined by the FSC/SSC phenotype and CD25 expression levels was determined by flow cytometric analysis. (B) The influence of AS601245 (10 μM) on CD3/CD28-induced cytokine expression (IL-2, IL-4, IL-6, IL-8, IL-17, TNF-α, and IFN-γ) was determined using BioPlex analysis. (C) Baseline and PMA-induced MHC-I and CD28 expression in Jurkat T cells was determined 48 h after the addition of AS601245 and PMA using flow cytometric analysis.