Fig 6.

Effect of AS601245 on JNK and JNK substrate activation. (A) Jurkat T cells and the latently HIV-1-infected CA5 T cells were stimulated with PMA, and changes in the JNK2 (upper bands) and JNK1 (lower bands) phosphorylation levels were determined by Western blotting using a phospho-JNK MAb. (B) CA5 T cells were stimulated with PMA in the absence or presence of AS601245, and activation of c-Jun, c-Fos, and JunB as possible JNK substrates was quantified using a TransAM assay (data are representative of results from two independent experiments).