Fig 5.

CD8⁺ T cell responses following DNA prime and different doses of viral vector boost. Mice were injected with 50 ng DREP.HIVconsv or 2.5 μg pTH.HIVconsv i.d. with EP and boosted 4 weeks later with one of three doses of MVA.HIVconsv: 5 × 10⁶ PFU (high), 5 × 10⁵ PFU (med), or 5 × 10⁴ PFU (low). Spleens were collected and analyzed 7 days later with IFN-γ ELISpot and with ICS for IFN-γ, TNF-α, and IL-2. Single, double, and triple denote how many of these cytokines the CD8⁺ T cells produced. Each group consisted of 5 mice. Results are displayed as group medians. Pairs of groups that were administered the same boost were compared statistically with the Mann-Whitney U test. In panel B, the total percentage of cytokine-positive cells was used for statistical comparison.