Fig 7.

pIL-27+G1F/M2 immunization prevented pulmonary pathology following RSV infection. (A) Lung histology was determined by H&E staining at 5 days postchallenge. An image for a representative animal is shown for each experimental group or for naïve mice (control). (B) Scores for pulmonary inflammation. Tissue sections obtained from each mouse were scored for inflammation on a scale of from 0 to 3 or 4 as described in Materials and Methods. (C) Relative expression of gob5 gene in lungs at 5 days postchallenge. Preimmunized mice were challenged with RSV A 3 weeks after the final immunization. Total RNAs were extracted from the lungs. The relative expression of gob5 mRNA was measured by qRT-PCR. Naïve mice neither immunized nor challenged were used as controls. Results are means ± SDs of 5 to 6 mice/group and are representative of two experiments.