Fig 1.

NK cell-mediated anti-HIV ADCC responses. (A) Gating was on CD3⁻ lymphocytes. Flow cytometry plots illustrate the gating strategy used to identify the anti-HIV ADCC responses of 3DL1⁺ and 3DL1⁻ NK cells. The gating strategy is illustrated for an HIV-negative donor and an HIV-infected donor. (B) Dot plots illustrate the requirement for HIV Env peptide and plasma-derived anti-HIV antibodies for NK cell activation in the whole-blood assay. (C) Dot plots depict a representative example of 3 experiments assessing the ability of NK cells within a whole-blood, HIV Env peptide, and HIV-infected plasma culture to express CD107a and produce IFN-γ, compared to enriched NK cells incubated with HIV Env peptides and HIV-infected plasma.